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positive selection macs separation kit  (Miltenyi Biotec)


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    Miltenyi Biotec positive selection macs separation kit
    Positive Selection Macs Separation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/positive+selection+macs+separation+kit/CD304+(BDCA-4%2FNeuropilin-1)+MicroBead+Kit%2C+human/pmc13130636-397-10-15
    Average 93 stars, based on 30 article reviews
    positive selection macs separation kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Multi-omics reveal vitamin D regulation of immune-gut microbiome interactions and tolerogenic pathways in inflammatory bowel disease
    Article Snippet: B cells were isolated using a negative selection MACS separation kit (Miltenyi B Cell Isolation Kit II, human, Catalog no. 130-091-151) according to manufacturer’s instructions. .. Plasmacytoid dendritic cells (pDCs) were isolated from PBMC using a positive selection MACS separation kit (Miltenyi CD304 BDCA-4/Neuropilin-1 MicroBead Kit, human, Catalog No. 130-090-532). .. Peripheral blood CD19 + B cells were co-cultured with pDC (5000 pDC: 250,000 B cell ratio) or without pDC in 6 well plates (culture media RPMI 1640 + 100 U/mL penicillin (ThermoFisher, Catalog No. 15140-122), 100 mg/mL streptomycin (ThermoFisher, Catalog No. 15140-122)+ 50 mg/mL gentamicin (ThermoFisher, Catalog No. 15750-060])+ FBS 2% with 100 nM of 1,25(OH)D2 (Sigma Aldrich, Catalog No. 740578) or vehicle (EtOH+ media) control for 48 h. F(ab') 2 Anti-Human IgM (10 μg/mL) (Jackson ImmunoResearch, Cat. No.109-006-129) was added to culture media for BCR stimulation.

    Article Title: Multi-omics reveal vitamin D regulation of immune-gut microbiome interactions and tolerogenic pathways in inflammatory bowel disease.
    Article Snippet: B cells were isolated using a negative selection MACS separation kit (Miltenyi B Cell Isolation Kit II, human, Catalog no. 130-091-151) according to manufacturer’s instructions. .. Plasmacytoid dendritic cells (pDCs) were isolated from PBMC using a positive selection MACS separation kit (Miltenyi CD304 BDCA-4/Neuropilin-1 MicroBead Kit, human, Catalog No. 130-090-532). .. Peripheral blood CD19+ B cells were co-cultured with pDC (5000 pDC: 250,000 B cell ratio) or without pDC in 6 well plates (culture media RPMI 1640 + 100 U/mL penicillin (ThermoFisher, Catalog No. 15140-122), 100 mg/mL streptomycin (ThermoFisher, Catalog No. 15140-122)+ 50 mg/mL gentamicin (ThermoFisher, Catalog No. 15750-060])+ FBS 2% with 100 nM of 1,25(OH)D2 (Sigma Aldrich, Catalog No. 740578) or vehicle (EtOH+ media) control for 48 h. F(ab’)2 Anti-Human IgM (10 μg/mL) (Jackson ImmunoResearch, Cat. No.109-006-129) was added to culture media for BCR stimulation.

    Selection:

    Article Title: Multi-omics reveal vitamin D regulation of immune-gut microbiome interactions and tolerogenic pathways in inflammatory bowel disease
    Article Snippet: B cells were isolated using a negative selection MACS separation kit (Miltenyi B Cell Isolation Kit II, human, Catalog no. 130-091-151) according to manufacturer’s instructions. .. Plasmacytoid dendritic cells (pDCs) were isolated from PBMC using a positive selection MACS separation kit (Miltenyi CD304 BDCA-4/Neuropilin-1 MicroBead Kit, human, Catalog No. 130-090-532). .. Peripheral blood CD19 + B cells were co-cultured with pDC (5000 pDC: 250,000 B cell ratio) or without pDC in 6 well plates (culture media RPMI 1640 + 100 U/mL penicillin (ThermoFisher, Catalog No. 15140-122), 100 mg/mL streptomycin (ThermoFisher, Catalog No. 15140-122)+ 50 mg/mL gentamicin (ThermoFisher, Catalog No. 15750-060])+ FBS 2% with 100 nM of 1,25(OH)D2 (Sigma Aldrich, Catalog No. 740578) or vehicle (EtOH+ media) control for 48 h. F(ab') 2 Anti-Human IgM (10 μg/mL) (Jackson ImmunoResearch, Cat. No.109-006-129) was added to culture media for BCR stimulation.

    Article Title: Multi-omics reveal vitamin D regulation of immune-gut microbiome interactions and tolerogenic pathways in inflammatory bowel disease.
    Article Snippet: B cells were isolated using a negative selection MACS separation kit (Miltenyi B Cell Isolation Kit II, human, Catalog no. 130-091-151) according to manufacturer’s instructions. .. Plasmacytoid dendritic cells (pDCs) were isolated from PBMC using a positive selection MACS separation kit (Miltenyi CD304 BDCA-4/Neuropilin-1 MicroBead Kit, human, Catalog No. 130-090-532). .. Peripheral blood CD19+ B cells were co-cultured with pDC (5000 pDC: 250,000 B cell ratio) or without pDC in 6 well plates (culture media RPMI 1640 + 100 U/mL penicillin (ThermoFisher, Catalog No. 15140-122), 100 mg/mL streptomycin (ThermoFisher, Catalog No. 15140-122)+ 50 mg/mL gentamicin (ThermoFisher, Catalog No. 15750-060])+ FBS 2% with 100 nM of 1,25(OH)D2 (Sigma Aldrich, Catalog No. 740578) or vehicle (EtOH+ media) control for 48 h. F(ab’)2 Anti-Human IgM (10 μg/mL) (Jackson ImmunoResearch, Cat. No.109-006-129) was added to culture media for BCR stimulation.

    Magnetic Cell Separation:

    Article Title: Multi-omics reveal vitamin D regulation of immune-gut microbiome interactions and tolerogenic pathways in inflammatory bowel disease
    Article Snippet: B cells were isolated using a negative selection MACS separation kit (Miltenyi B Cell Isolation Kit II, human, Catalog no. 130-091-151) according to manufacturer’s instructions. .. Plasmacytoid dendritic cells (pDCs) were isolated from PBMC using a positive selection MACS separation kit (Miltenyi CD304 BDCA-4/Neuropilin-1 MicroBead Kit, human, Catalog No. 130-090-532). .. Peripheral blood CD19 + B cells were co-cultured with pDC (5000 pDC: 250,000 B cell ratio) or without pDC in 6 well plates (culture media RPMI 1640 + 100 U/mL penicillin (ThermoFisher, Catalog No. 15140-122), 100 mg/mL streptomycin (ThermoFisher, Catalog No. 15140-122)+ 50 mg/mL gentamicin (ThermoFisher, Catalog No. 15750-060])+ FBS 2% with 100 nM of 1,25(OH)D2 (Sigma Aldrich, Catalog No. 740578) or vehicle (EtOH+ media) control for 48 h. F(ab') 2 Anti-Human IgM (10 μg/mL) (Jackson ImmunoResearch, Cat. No.109-006-129) was added to culture media for BCR stimulation.

    Article Title: Multi-omics reveal vitamin D regulation of immune-gut microbiome interactions and tolerogenic pathways in inflammatory bowel disease.
    Article Snippet: B cells were isolated using a negative selection MACS separation kit (Miltenyi B Cell Isolation Kit II, human, Catalog no. 130-091-151) according to manufacturer’s instructions. .. Plasmacytoid dendritic cells (pDCs) were isolated from PBMC using a positive selection MACS separation kit (Miltenyi CD304 BDCA-4/Neuropilin-1 MicroBead Kit, human, Catalog No. 130-090-532). .. Peripheral blood CD19+ B cells were co-cultured with pDC (5000 pDC: 250,000 B cell ratio) or without pDC in 6 well plates (culture media RPMI 1640 + 100 U/mL penicillin (ThermoFisher, Catalog No. 15140-122), 100 mg/mL streptomycin (ThermoFisher, Catalog No. 15140-122)+ 50 mg/mL gentamicin (ThermoFisher, Catalog No. 15750-060])+ FBS 2% with 100 nM of 1,25(OH)D2 (Sigma Aldrich, Catalog No. 740578) or vehicle (EtOH+ media) control for 48 h. F(ab’)2 Anti-Human IgM (10 μg/mL) (Jackson ImmunoResearch, Cat. No.109-006-129) was added to culture media for BCR stimulation.



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